C5 Mutation in Japanese Patients with an unhealthy Response We amplified all 41 exons of the C5 gene with primer models specifically designed for each exon, using as a template genomic DNA prepared from mononuclear cells acquired from sufferers who had an unhealthy response, and we directly sequenced all of the PCR items from each exon . We identified a single missense C5 heterozygous mutation at exon 21, c.Arg885His, in both patients who had a poor response. This mutation was not seen in 7 patients who had an excellent response to eculizumab .Try to be more active physically Speaking of fitness level, you could also be happy to know that certain pains could be relieved by performing more physical exercises on a regular basis. You definitely know that any kind of sport is actually a goldmine for your wellbeing and you are highly encouraged to be more physically active every day. Just visit a gym for some weeks or teach in the comfort and ease of your house and you may quickly see that a lot of pains will go away.